Fey P D, Ulphani J S, Götz F, Heilmann C, Mack D, Rupp M E
Division of Infectious Disease, University of Nebraska Medical Center, Omaha, NE 68198-5400, USA.
J Infect Dis. 1999 Jun;179(6):1561-4. doi: 10.1086/314762.
To determine whether a relationship exists between biofilm formation and hemagglutination in Staphylococcus epidermidis, 20 skin isolates and 19 prosthetic valve endocarditis isolates were characterized for biofilm formation, hemagglutination, and the presence of a 357-bp polymerase chain reaction product within icaA. A strong association existed between biofilm formation, which has been linked to strains that produce polysaccharide intercellular adhesin (PIA), and hemagglutination. Strains that produced biofilm were significantly (P<.001) more likely to mediate hemagglutination (16 biofilm-positive/hemagglutination-positive strains and 19 biofilm-negative/hemagglutination-negative strains) within the 39 clinical strains tested. In addition, Staphylococcus carnosus TM300, a biofilm-negative, hemagglutination-negative strain, carrying the ica operon-containing plasmid pCN27, produced significant biofilm on glass and mediated hemagglutination (>/=1/128). It was concluded that production of PIA and hemagglutination are strongly associated and that PIA, at least in part, mediates hemagglutination in S. epidermidis.
为确定表皮葡萄球菌生物膜形成与血细胞凝集之间是否存在关联，对20株皮肤分离株和19株人工瓣膜心内膜炎分离株进行了生物膜形成、血细胞凝集以及icaA基因内一段357 bp聚合酶链反应产物存在情况的特性分析。已证实与产生胞间多糖黏附素（PIA）的菌株相关的生物膜形成与血细胞凝集之间存在强关联。在所检测的39株临床菌株中，形成生物膜的菌株介导血细胞凝集的可能性显著更高（P<0.001）（16株生物膜阳性/血细胞凝集阳性菌株和19株生物膜阴性/血细胞凝集阴性菌株）。此外，携带含ica操纵子的质粒pCN27的肉葡萄球菌TM300，一株生物膜阴性、血细胞凝集阴性的菌株，在玻璃上形成了显著的生物膜并介导了血细胞凝集（≥1/128）。得出的结论是，PIA的产生与血细胞凝集密切相关，并且PIA至少部分介导了表皮葡萄球菌的血细胞凝集。
