结核分枝杆菌主要细胞分裂基因ftsZ的转录分析。
Transcriptional analysis of the principal cell division gene, ftsZ, of Mycobacterium tuberculosis.
作者信息
Roy Sougata, Ajitkumar Parthasarathi
机构信息
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore-560012, India.
出版信息
J Bacteriol. 2005 Apr;187(7):2540-50. doi: 10.1128/JB.187.7.2540-2550.2005.
Abstract
Multiple promoters drive the expression of the principal cell division gene, ftsZ, in bacterial systems. Primer extension analysis of total RNA from Mycobacterium tuberculosis and a Mycobacterium smegmatis transformant containing 1.117 kb of the upstream region of M. tuberculosis ftsZ and promoter fusion studies identified six ftsZ transcripts and their promoters in the ftsQ open reading frame and ftsQ-ftsZ intergenic region. The presence of multiple promoters reflects the requirement to maintain a high basal level of, or to differentially regulate, FtsZ expression during different growth conditions of the pathogen in vivo.
摘要
多个启动子驱动细菌系统中主要细胞分裂基因ftsZ的表达。对来自结核分枝杆菌的总RNA以及含有结核分枝杆菌ftsZ上游区域1.117 kb的耻垢分枝杆菌转化体进行引物延伸分析，并开展启动子融合研究，在ftsQ开放阅读框和ftsQ-ftsZ基因间区域鉴定出6种ftsZ转录本及其启动子。多个启动子的存在反映了在病原体体内不同生长条件下维持FtsZ高基础水平表达或进行差异调节的需求。
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