Bao Xin-min, Shu Si-yun, Wang Hong
Institute for Neuroscience of the First Military Medical University, Zhujiang Hospital, Guangzhou 510282, China.
Chin Med J (Engl). 2005 Mar 5;118(5):398-403.
A new brain region, the marginal division (MrD), was discovered at the caudal margin of the neostriatum. The MrD was shown to be involved in learning and memory in the rat. The aim of this study was to investigate the expression of the immediate-early genes c-fos and c-jun in the MrD of the striatum during learning and memory processes in the rat, immunocytochemical and Western blot methods were used to examine Y-maze trained rats.
The rats were divided into three groups, namely the training, pseudotraining, and control groups. After Y-maze training, the expression of the immediate-early genes c-fos and c-jun in the MrD of the rats was investigated using immunocytochemical and Western blot methods.
After one hour of Y-maze training, the expression of c-jun and c-fos proteins was significantly enhanced in the MrD; the c-jun protein, in particular, was more intensely expressed in this region than in other parts of the striatum. The expression of these two proteins in the training group was significantly higher than in the pseudotraining and control groups. In addition, positive expression was also found in the hippocampus, cingulum cortex, thalamus, and in other areas. Western blot disclosed two immunoreactive bands for the anti-c-fos antibody (47 kD and 54 kD) and two immunoreactive bands for the anti-c-jun antibody (39 kD and 54 kD).
These results indicate that the immediate-early genes c-fos and c-jun participate in signal transduction during the learning and memory processes associated with Y-maze training in rats.
一个新的脑区——边缘区（MrD），在新纹状体的尾侧边缘被发现。研究表明，MrD参与大鼠的学习和记忆过程。本研究旨在探讨大鼠学习和记忆过程中，纹状体MrD中即早基因c-fos和c-jun的表达情况，采用免疫细胞化学和蛋白质印迹法检测经Y迷宫训练的大鼠。
将大鼠分为三组，即训练组、假训练组和对照组。经Y迷宫训练后，采用免疫细胞化学和蛋白质印迹法检测大鼠MrD中即早基因c-fos和c-jun的表达。
Y迷宫训练1小时后，MrD中c-jun和c-fos蛋白的表达显著增强；尤其是c-jun蛋白，在该区域的表达比纹状体其他部位更为强烈。训练组中这两种蛋白的表达显著高于假训练组和对照组。此外，在海马、扣带回皮质、丘脑及其他区域也发现了阳性表达。蛋白质印迹法显示，抗c-fos抗体有两条免疫反应带（47 kD和54 kD），抗c-jun抗体有两条免疫反应带（39 kD和54 kD）。
这些结果表明，即早基因c-fos和c-jun参与大鼠Y迷宫训练相关学习和记忆过程中的信号转导。
