De Haro Luciano, Janknecht Ralf
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA.
Genomics. 2005 Apr;85(4):493-502. doi: 10.1016/j.ygeno.2004.12.003.
The ER71 protein belongs to the ETS transcription factor family and is testis-specifically expressed in adult mice. Here we describe the cloning of the respective Etsrp71 gene and promoter. The murine Etsrp71 gene is relatively compact, spanning 3 kb, and is arranged into seven exons and six introns, the majority of which are highly conserved in rat and human. Its promoter is devoid of a TATA box and transcription starts at multiple sites. Furthermore, two ER71 isoforms exist that differ by 22 N-terminal amino acids, but show no difference in DNA binding or transactivation. Close to the transcription initiation sites, we identified a binding site for the transcription factor Sp1. Mutation of this binding site severely diminished the ability of Sp1 to activate the Etsrp71 promoter. The findings reported here may provide avenues for further research elucidating the regulation of Etsrp71 gene activity during embryogenesis and in adult testes.
ER71蛋白属于ETS转录因子家族，在成年小鼠睾丸中特异性表达。在此，我们描述了相应的Etsrp71基因及其启动子的克隆。小鼠Etsrp71基因相对紧凑，跨度为3 kb，由7个外显子和6个内含子组成，其中大部分在大鼠和人类中高度保守。其启动子缺乏TATA框，转录起始于多个位点。此外，存在两种ER71亚型，它们在N端有22个氨基酸的差异，但在DNA结合或反式激活方面没有差异。在转录起始位点附近，我们鉴定出一个转录因子Sp1的结合位点。该结合位点的突变严重削弱了Sp1激活Etsrp71启动子的能力。本文报道的研究结果可能为进一步研究阐明胚胎发育和成年睾丸中Etsrp71基因活性的调控提供途径。
