Gupta Smriti, Wang Bing-Bing, Stryker Gabrielle A, Zanetti María Eugenia, Lal Shailesh K
Department of Biological Sciences, Oakland University, Rochester, MI 48309-4401, USA.
Biochim Biophys Acta. 2005 May 1;1728(3):105-14. doi: 10.1016/j.bbaexp.2005.01.004. Epub 2005 Feb 23.
The serine-arginine (SR)-rich splicing proteins are highly conserved RNA binding nuclear phosphor-proteins that play important roles in both regular and alternative splicing. Here we describe two novel putative SR genes from maize, designated zmRSp31A and zmRSp31B. Both genes contain characteristic RNA binding motifs RNP-1 and RNP-2, a serine/arginine-rich (RS) domain and share significant sequence similarity to the Arabidopsis atRSp31 family of SR proteins. Both zmRSp31A and zmRSp31B produce multiple transcripts by alternative splicing, of which majority of the alternatively spliced transcripts utilize non-canonical splice sites. zmRSp31A and zmRSp31B produce at least six and four transcripts, respectively, of which only one corresponds to the wild type proteins for each gene. All the alternatively spliced transcripts of both the genes, with one exception, are predicted to encode small truncated proteins containing only the RNP-2 domain of their first RNA recognition motif and completely lack the carboxyl terminal RS domain. We provide evidence that some of the alternatively spliced transcripts of both genes are associated with polysomes and interact with the translational machinery.
富含丝氨酸 - 精氨酸（SR）的剪接蛋白是高度保守的RNA结合核磷蛋白，在常规剪接和可变剪接中均发挥重要作用。在此，我们描述了来自玉米的两个新的假定SR基因，命名为zmRSp31A和zmRSp31B。这两个基因都含有特征性的RNA结合基序RNP - 1和RNP - 2、一个富含丝氨酸/精氨酸（RS）的结构域，并且与拟南芥的SR蛋白家族atRSp31具有显著的序列相似性。zmRSp31A和zmRSp31B均通过可变剪接产生多种转录本，其中大多数可变剪接转录本利用非经典剪接位点。zmRSp31A和zmRSp31B分别产生至少六种和四种转录本，其中每个基因只有一种对应野生型蛋白。这两个基因的所有可变剪接转录本（有一个例外）预计编码仅包含其第一个RNA识别基序的RNP - 2结构域且完全缺乏羧基末端RS结构域的小截短蛋白。我们提供证据表明，这两个基因的一些可变剪接转录本与多核糖体相关并与翻译机制相互作用。
