成人蛔虫、犊蛔虫和犬弓首蛔虫的蛋白质色谱研究。
Protein chromatographic study on adult Ascaris lumbricoides, Ascaris vitulorum and Toxocara canis.
作者信息
Safar E H, el-Rifaei F, Maklad K M
机构信息
Research Institute of Ophthalmology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
出版信息
J Egypt Soc Parasitol. 1992 Apr;22(1):171-6.
Chromatographic analysis using Sephacryl S-300 was done on protein extract of adult Ascaris lumbricoides, A. vitulorum and Toxocara canis worms. Six fractions were separated from both Ascaris species and nine from T. canis. Fractions revealed a variety of molecular weights in relation to the three species of worms that ranged from 6,000-70,000 daltons. Crude extract of T. canis showed cross reaction with both species of Ascaris sera using double gel diffusion, while no reaction was obtained using a partially purified antigen.
使用Sephacryl S - 300对成年蛔虫、牛蛔虫和犬弓首蛔虫的蛋白质提取物进行了色谱分析。从两种蛔虫中分离出六个组分，从犬弓首蛔虫中分离出九个组分。这些组分显示出与这三种蠕虫相关的多种分子量，范围为6000 - 70000道尔顿。犬弓首蛔虫的粗提物在双向凝胶扩散试验中与两种蛔虫血清均呈交叉反应，而使用部分纯化抗原时未获得反应。
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