Inwoley K A, Sawadogo D, Mizero L, Salou M, Karim N, Sangaré A
Département d'hématologie, d'immunologie et de biologie cellulaire, Unité de formation et de recherche des sciences pharmaceutiques et biologiques, Université de Cocody, Abidjan, Côte d'Ivoire.
Bull Soc Pathol Exot. 2004;97(5):319-22.
Flow cytometry is nowadays the first-line method for immunophenotypic identification of blast cells but is not so usual in limited-resources countries. We have investigated on the usefulness of this tool in Abidjan, Côte d'Ivoire. Bone marrow sample from 13 patients with acute leukemia identified by cytology and cytochemical analysis was immunophenotyped by using monoclonal antibodies directed to: T lymphoid cells (CD3, CD5, CD7); B lymphoid cells (CD10, CD19, CD20, CD22, HLA-DR) and myeloid cells (CD13, CD33). Immunophenotyping allowed us to confirm the diagnosis of 6 de novo acute leukemias (2 acute myeloid leukaemias, 4 acute lymphoid leukemias) and 7 acute leukaemias resulting from chronic myeloid leukaemias. Immunophenotyping also characterizes the atypical/aberrant lineage essential for the prognosis: 2 biphenotypic acute leukemias (myeloid/lymphoid T) were identified. Our results suggest that flow cytometry may be a useful additional tool to identify the specific leukemic cell, to make a better classification as well as a prognosis evaluation of patients with acute leukemias.
如今，流式细胞术是原始细胞免疫表型鉴定的一线方法，但在资源有限的国家并不常用。我们在科特迪瓦的阿比让研究了该工具的实用性。通过细胞学和细胞化学分析确定的13例急性白血病患者的骨髓样本，使用针对以下细胞的单克隆抗体进行免疫表型分析：T淋巴细胞（CD3、CD5、CD7）；B淋巴细胞（CD10、CD19、CD20、CD22、HLA-DR）和髓系细胞（CD13、CD33）。免疫表型分析使我们能够确诊6例初发急性白血病（2例急性髓细胞白血病，4例急性淋巴细胞白血病）和7例由慢性髓细胞白血病导致的急性白血病。免疫表型分析还可对预后至关重要的非典型/异常谱系进行特征描述：识别出2例双表型急性白血病（髓系/T淋巴细胞）。我们的结果表明，流式细胞术可能是一种有用的辅助工具，可用于识别特定的白血病细胞，对急性白血病患者进行更好的分类和预后评估。
