红细胞：顺式和反式环氧二十碳三烯酸的储存库。
Red blood cells: reservoirs of cis- and trans-epoxyeicosatrienoic acids.
作者信息
Jiang Houli, Quilley John, Reddy L Manmohan, Falck John R, Wong Patrick Y K, McGiff John C
机构信息
Department of Pharmacology, New York Medical College, Valhalla, NY 10595, USA.
出版信息
Prostaglandins Other Lipid Mediat. 2005 Jan;75(1-4):65-78. doi: 10.1016/j.prostaglandins.2004.10.003.
Epoxyeicosatrienoic acids (EETs) are candidate endothelium-derived hyperpolarizing factors that demonstrate a wide range of biological effects. The presence of both cis- and trans-EETs in rat plasma was identified with HPLC-electrospray ionization tandem mass spectrometry in this study. The total EETs in plasma are 38.2 ng/ml with cis-EETs representing 21.4 +/- 0.4 ng/ml and trans-EETs 16.8 +/- 0.4 ng/ml. EETs in RBCs were estimated to be 20.2 ng/10(9) RBCs, which corresponds to 200 ng in RBCs contained in 1 ml blood. RBC incubation with 10 mM tert-butyl hydroperoxide resulted in 4.4-fold increase of total cis-EETs (from 9.2 to 40.2 ng/10(9) RBCs) and 5.5-fold increase of total trans-EETs (from 11.0 to 60.8 ng/10(9) RBCs). EETs were released (2 ng/ml) from RBCs after incubation at 37 degrees C for 10 min even after being washed 3 times, indicating that RBCs are reservoirs of plasma EETs. The identification of cis- and trans-EETs in RBCs and in plasma as well as their release from RBCs suggest a vasoregulatory role of RBCs in view of their potent vasoactivity.
环氧二十碳三烯酸（EETs）是一类候选的内皮源性超极化因子，具有广泛的生物学效应。本研究采用高效液相色谱-电喷雾电离串联质谱法鉴定了大鼠血浆中顺式和反式EETs的存在。血浆中总EETs含量为38.2 ng/ml，其中顺式EETs为21.4±0.4 ng/ml，反式EETs为16.8±0.4 ng/ml。红细胞中EETs含量估计为20.2 ng/10⁹个红细胞，相当于1 ml血液中红细胞所含的200 ng。用10 mM叔丁基过氧化氢孵育红细胞后，总顺式EETs增加了4.4倍（从9.2 ng/10⁹个红细胞增加到40.2 ng/10⁹个红细胞），总反式EETs增加了5.5倍（从11.0 ng/10⁹个红细胞增加到60.8 ng/10⁹个红细胞）。即使经过3次洗涤，红细胞在37℃孵育10分钟后仍会释放出EETs（2 ng/ml），这表明红细胞是血浆EETs的储存库。红细胞和顺式及反式EETs在血浆中的鉴定以及它们从红细胞中的释放，鉴于其强大的血管活性，提示红细胞在血管调节中发挥作用。
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