Roux Aurélien, Cuvelier Damien, Nassoy Pierre, Prost Jacques, Bassereau Patricia, Goud Bruno
UMR 144 CNRS/Institut Curie, Paris, France.
EMBO J. 2005 Apr 20;24(8):1537-45. doi: 10.1038/sj.emboj.7600631. Epub 2005 Mar 24.
We have recently developed a minimal system for generating long tubular nanostructures that resemble tubes observed in vivo with biological membranes. Here, we studied membrane tube pulling in ternary mixtures of sphingomyelin, phosphatidylcholine and cholesterol. Two salient results emerged: the lipid composition is significantly different in the tubes and in the vesicles; tube fission is observed when phase separation is generated in the tubes. This shows that lipid sorting may depend critically on both membrane curvature and phase separation. Phase separation also appears to be important for membrane fission in tubes pulled out of giant liposomes or purified Golgi membranes.
我们最近开发了一种最小系统,用于生成类似于体内观察到的带有生物膜的长管状纳米结构。在此,我们研究了鞘磷脂、磷脂酰胆碱和胆固醇三元混合物中的膜管拉伸。出现了两个显著结果:管状物和囊泡中的脂质组成有显著差异;当管状物中产生相分离时,会观察到管裂变。这表明脂质分选可能严重依赖于膜曲率和相分离。相分离对于从巨型脂质体或纯化的高尔基体膜中拉出的管状物中的膜裂变似乎也很重要。