Huang Tianhui, Shen Pingniang, Shen Yongjia
Institute of Chemical and Pharmaceutical, East China University of Science and Technology, 897 #, 130 Meilong Road, Shanghai 200237, China.
J Chromatogr A. 2005 Feb 25;1066(1-2):239-42. doi: 10.1016/j.chroma.2005.01.025.
High-speed counter-current chromatography (HSCCC) was successfully applied to the preparative separation and purification of deoxyschisandrin and gamma-schisandrin from the crude petroleum ether extracts of Schisandra chinensis (Turcz.) Baill. The optimum solvent system composed of n-hexane-methanol-water (35:30:3, v/v) led to the successful preparation of deoxyschisandrin and gamma-schisandrin. The analysis of HPLC for each peak fraction of preparative HSCCC showed that the purity of deoxyschisandrin (8 mg) was over 98% and gamma-schisandrin (12 mg) was over 96% from 100 mg of the crude petroleum ether extracts in one-step separation.
高速逆流色谱法（HSCCC）成功应用于从五味子（Schisandra chinensis (Turcz.) Baill.）石油醚粗提物中制备分离和纯化五味子醇甲和γ-五味子素。由正己烷-甲醇-水（35:30:3，v/v）组成的最佳溶剂系统成功制备出了五味子醇甲和γ-五味子素。对制备型HSCCC各峰馏分的HPLC分析表明，从100 mg石油醚粗提物一步分离得到的8 mg五味子醇甲纯度超过98%，12 mgγ-五味子素纯度超过96%。
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