Nakamura Y, Tonomura Y, Hagihara B
J Biochem. 1979 Aug;86(2):443-6. doi: 10.1093/oxfordjournals.jbchem.a132542.
Solubilized sarcoplasmic reticulum (SSR) was prepared by solubilizing fragmented sarcoplasmic reticulum (FSR) with a nonionic detergent (C12E8) then displacing the detergent with Tween 80, using a DEAE-cellulose column. The UV absorption of SSR decreased reversibly at about 286 and 292 nm on removal of free Ca2+ ions, while no change in the fluorescence spectrum was detectable. On the other hand, the fluorescence intensity of FSR decreased 3-4% on removal of free Ca2+ ions, as previously reported by Dupont [(1976) Biochem. Biophys. Res. Commun. 71, 544-550]. The UV absorption of FSR increased reversibly at about 270-280 nm on removal of free Ca2+ ions, but the rate of the change was very slow (k = about 0.1 min-1).
通过用非离子洗涤剂（C12E8）溶解破碎的肌浆网（FSR），然后使用DEAE-纤维素柱用吐温80置换洗涤剂，制备了可溶性肌浆网（SSR）。去除游离Ca2+离子后，SSR在约286和292nm处的紫外吸收可逆下降，而荧光光谱未检测到变化。另一方面，如杜邦先前报道的[(1976)生物化学与生物物理研究通讯71, 544 - 550]，去除游离Ca2+离子后，FSR的荧光强度下降3 - 4%。去除游离Ca2+离子后，FSR在约270 - 280nm处的紫外吸收可逆增加，但变化速率非常缓慢（k约为0.1 min-1）。
