Huang Gang-Liang, Liu Tian-Cai, Liu Man-Xi, Mei Xin-Ya
Key Laboratory of Biomedical Photonics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China.
Anal Biochem. 2005 May 1;340(1):52-6. doi: 10.1016/j.ab.2005.01.013.
A sensitive, specific, and rapid method for the detection of carbohydrate-protein interactions was demonstrated using quantum dots (QDs) as a fluorescence label coupled with protein. 1,3-Dipolar cycloaddition between azide and alkyne was exploited to attach alpha-d-glucopyranoside to a C(14) hydrocarbon chain that noncovalently binds to the microtiter well surface, and the product formation was detected by both electrospray ionization-mass spectrometry (ESI-MS) and QD- (or fluorescein isothiocyanate (FITC))-conjugated lectin binding. It indicated that the peak intensity of the fluorescence emission was proportional to the initial concanavalin A (Con A) concentration in the range of 2 x 10(-3) micromol/L to 2 x 10(-2)mmol/L with a detection limit at least 100 times lower than that of the FITC-based method.
一种灵敏、特异且快速的检测碳水化合物 - 蛋白质相互作用的方法得以展示，该方法使用量子点（QDs）作为与蛋白质偶联的荧光标记。利用叠氮化物和炔烃之间的1,3 - 偶极环加成反应，将α - D - 吡喃葡萄糖苷连接到非共价结合于微孔板表面的C(14)烃链上，通过电喷雾电离质谱（ESI - MS）以及量子点（或异硫氰酸荧光素（FITC））偶联的凝集素结合来检测产物形成。结果表明，在2×10⁻³ μmol/L至2×10⁻² mmol/L范围内，荧光发射的峰值强度与初始伴刀豆球蛋白A（Con A）浓度成正比，其检测限至少比基于FITC的方法低100倍。
