Liao Ping, Yan Zheng-Yu, Xu Zhi-Ji, Sun Xiao
Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2009 Jun;72(5):1066-70. doi: 10.1016/j.saa.2008.12.039. Epub 2009 Jan 4.
Aqueous thiol-capped CdSe QDs with a narrow, symmetric emission were prepared under a low temperature. Based on the fluorescence enhancement of thiol-stabilized CdSe quantum dots (QDs) caused by edaravone, a simple, rapid and specific quantitative method was proposed to the edaravone determination. The concentration dependence of fluorescence intensity followed the binding of edaravone to surface of the thiol-capped CdSe QDs was effectively described by a modified Langmuir-type binding isotherm. Factors affecting the fluorescence detection for edaravone with thiol-stabilized CdSe QDs were studied, such as the effect of pH, reaction time, the concentration of CdSe QDs and so on. Under the optimal conditions, the calibration plot of C/(I-I(0)) with concentration of edaravone was linear in the range of (1.45-17.42) microg/mL (0.008-0.1 micromol/L) with correlation coefficient of 0.998. The limit of detection (LOD) (3sigma/kappa) was 0.15 microg/mL (0.0009 micromol/mL). Possible interaction mechanism was discussed.
在低温条件下制备了具有窄而对称发射峰的水相巯基封端的CdSe量子点。基于依达拉奉引起的巯基稳定的CdSe量子点（QDs）荧光增强，提出了一种简单、快速且特异的依达拉奉定量测定方法。通过修正的朗缪尔型结合等温线有效地描述了荧光强度与依达拉奉在巯基封端的CdSe量子点表面结合的浓度依赖性。研究了影响巯基稳定的CdSe量子点对依达拉奉荧光检测的因素，如pH值、反应时间、CdSe量子点浓度等。在最佳条件下，依达拉奉浓度与C/(I-I(0))的校准曲线在(1.45 - 17.42)μg/mL（0.008 - 0.1μmol/L）范围内呈线性，相关系数为0.998。检测限（LOD）（3σ/k）为0.15μg/mL（0.0009μmol/mL）。并讨论了可能的相互作用机制。
