Sekiya Nobuyasu, Hikiami Hiroaki, Yokoyama Koichi, Kouta Kazufumi, Sakakibara Iwao, Shimada Yutaka, Terasawa Katsutoshi
Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Biol Pharm Bull. 2005 Apr;28(4):667-70. doi: 10.1248/bpb.28.667.
Crude preparations of Stephania tetrandra S. MOORE (ST), a traditional herbal medicine, have been used safely for arthritis and silicosis in China. In this study, we demonstrated that ST in vitro protects red blood cells from 2,2-azo-bis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis. The inhibitory effect was dose-dependent at concentrations of 10 to 1000 microg/ml. Moreover, tests were carried out to identify the main ingredient of ST that exerts a scavenging effect on free-radicals. Three representative alkaloids, tetrandrine, fangchinoline, and cyclanoline, isolated from ST, were found to have inhibitory activities against AAPH-induced lysis of red blood cells (RBC). Furthermore, the ingestion of 200 mg of ST extract was associated with a significant increase in free-radical scavenging effect of plasma in rats. These results suggest that ST as antioxidant inhibits AAPH-induced hemolysis of RBC both in vitro and in vivo.
传统草药粉防己(Stephania tetrandra S. MOORE,ST)的粗制剂在中国已被安全用于治疗关节炎和矽肺病。在本研究中,我们证明ST在体外可保护红细胞免受2,2-偶氮二(2-脒基丙烷)二盐酸盐(AAPH)诱导的溶血作用。在10至1000微克/毫升的浓度范围内,抑制作用呈剂量依赖性。此外,还进行了试验以确定ST中对自由基具有清除作用的主要成分。从ST中分离出的三种代表性生物碱,粉防己碱、防己诺林碱和轮环藤酚碱,被发现对AAPH诱导的红细胞(RBC)裂解具有抑制活性。此外,摄入200毫克ST提取物与大鼠血浆自由基清除作用的显著增加有关。这些结果表明,作为抗氧化剂的ST在体外和体内均能抑制AAPH诱导的RBC溶血。