Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, Wuhan 430071, China.
J Pharm Biomed Anal. 2012 Mar 5;61:252-5. doi: 10.1016/j.jpba.2011.11.027. Epub 2011 Dec 6.
A simple and selective method was developed for the simultaneous determination of tetrandrine and fangchinoline in herbal medicine by HPLC with electrochemical detection (ECD) on a bare glassy carbon electrode. The HPLC separation and ECD conditions have been optimized. The separation was carried out on a WondaSil C18-WR column (4.6 mm × 250 mm, 5 μm), with the mobile phase of acetonitrile-ammonium acetate buffer (pH 6.5; 40 mM) (32:68, v/v) using an isocratic elution at the flow rate of 0.5 mL/min. The electrochemical detection potential was set at +0.9 V. The obtained LODs for tetrandrine and fangchinoline were 0.26 and 0.27 μmol/L, respectively. The method was successfully applied to the analysis of tetrandrine and fangchinoline contents in Stephania tetrandra S. Moore. It has been demonstrated that the LC-ECD method is an excellent technique for analysis of the herbal medicine. The mean recoveries were in the range of 95-105%, while the precision expressed as repetition of peak area was lower than 2.7%.
建立了一种在裸玻碳电极上用高效液相色谱电化学检测（HPLC-ECD）同时测定中草药中粉防己碱和防己诺林碱的方法。优化了 HPLC 分离和 ECD 条件。在 WondaSil C18-WR 柱（4.6mm×250mm，5μm）上，以乙腈-乙酸铵缓冲液（pH6.5；40mM）（32:68，v/v）为流动相，流速为 0.5mL/min，采用等度洗脱进行分离。电化学检测电位设置为+0.9V。粉防己碱和防己诺林碱的检出限分别为 0.26 和 0.27μmol/L。该方法成功应用于测定粉防己和粉防己碱的含量。结果表明，LC-ECD 法是分析中草药的一种很好的技术。平均回收率在 95-105%之间，而峰面积重复的精密度低于 2.7%。
