Tanaka M, Sasaki T
Dept. of Experimental Therapeutics, Kanazawa University, Japan.
Gan To Kagaku Ryoho. 1992 May;19(5):743-8.
The usefulness of in vivo-like culture system using a specialized collagen gel matrix (Spongostan) derived from pigskin was summarized. In this culture system, the in vivo properties maintained in vitro, showing three-dimensional growth; maintenance of tissue architecture. This culture system was applied for the chemosensitivity test using human cancer specimens. There was a close association between the results of this assay and those of the nude mouse assay. Moreover, this system could be useful for the prediction of drug sensitivity against human cancers obtained from a surgery and biopsy, including scirrhous type gastric carcinomas. This in vitro assay system seems to be a promising method for chemosensitivity test, because of its rapidity and convenience.
总结了使用源自猪皮的特殊胶原凝胶基质（海绵明胶）的类体内培养系统的实用性。在该培养系统中，体外维持了体内特性，呈现三维生长；组织结构得以维持。该培养系统应用于使用人类癌症标本的化学敏感性测试。该检测结果与裸鼠检测结果之间存在密切关联。此外，该系统对于预测从手术和活检获得的人类癌症（包括硬癌型胃癌）的药物敏感性可能有用。由于其快速性和便利性，这种体外检测系统似乎是一种很有前景的化学敏感性测试方法。
