Kobayashi Hisayuki
Biochemical Laboratory, Nitta Gelatin Inc., Futamata, Yao-City, Japan.
Methods Mol Med. 2005;110:59-67. doi: 10.1385/1-59259-869-2:059.
For effective cancer chemotherapy, chemosensitivity testing of anticancer drugs should be performed using fresh surgical specimens obtained from the cancer. We have developed a new in vitro chemosensitivity test named the collagen gel droplet embedded culture drug sensitivity test (CD-DST). The CD-DST method consists of a collagen gel droplet embedded culture step, exposure and washout of anticancer drug, a serum-free culture step, and evaluation of anticancer effect by image analysis. This method has many advantages including a high success rate for primary culture, the need for only a small number of cells for the test, easy quantification of the anticancer effects without contamination with fibroblasts by using an image analysis system, a good correlation between in vitro and in vivo results, and simplicity and speed. The CD-DST method can be performed in the laboratory using a system kit Primaster.
为实现有效的癌症化疗，应使用从癌症患者获取的新鲜手术标本进行抗癌药物的化学敏感性测试。我们开发了一种新的体外化学敏感性测试方法，称为胶原凝胶微滴包埋培养药物敏感性测试（CD-DST）。CD-DST方法包括胶原凝胶微滴包埋培养步骤、抗癌药物的暴露和洗脱、无血清培养步骤以及通过图像分析评估抗癌效果。该方法具有许多优点，包括原代培养成功率高、测试仅需少量细胞、使用图像分析系统易于对抗癌效果进行定量且不受成纤维细胞污染、体外和体内结果具有良好的相关性以及简单快速。CD-DST方法可在实验室使用Primaster系统试剂盒进行操作。
