使用聚合酶链反应（PCR）诊断患有严重玻璃体炎的眼睛中的弓形虫性脉络膜视网膜炎。

Use of PCR to diagnose Toxoplasma gondii chorioretinitis in eyes with severe vitritis.

作者信息

Okhravi Narciss, Jones Colin D, Carroll Nora, Adamson Peter, Luthert Philip, Lightman Susan

机构信息

Department of Clinical Ophthalmology, Institute of Ophthalmology, Moorfields Eye Hospital, London, UK.

出版信息

Clin Exp Ophthalmol. 2005 Apr;33(2):184-7. doi: 10.1111/j.1442-9071.2005.00992.x.

DOI:10.1111/j.1442-9071.2005.00992.x

PMID:15807829

Abstract

Two cases are reported of intraocular inflammation in which severe vitritis hampered the fundal view, making an accurate clinical diagnosis impossible, and vitreous analysis using conventional techniques was unhelpful. PCR for Toxoplasma gondii was positive in both cases and provided the only way of confirming the diagnosis. Other ocular samples also underwent PCR for Toxoplasma DNA and the specificity of this approach is demonstrated.

摘要

报告了两例眼内炎症病例，其中严重的玻璃体炎妨碍了眼底观察，无法进行准确的临床诊断，并且使用传统技术进行玻璃体分析也无济于事。两例病例中弓形虫的聚合酶链反应（PCR）均为阳性，这是确诊的唯一方法。其他眼部样本也进行了弓形虫DNA的PCR检测，并证明了这种方法的特异性。