特异性抗原刺激对上皮内淋巴细胞向小肠黏膜迁移的影响。
Effect of specific antigen stimulation on intraepithelial lymphocyte migration to small intestinal mucosa.
作者信息
Komoto S, Miura S, Koseki S, Goto M, Hachimura S, Fujimori H, Hokari R, Hara Y, Ogino T, Watanabe C, Nagata H, Kaminogawa S, Hibi T, Ishii H
机构信息
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
出版信息
Clin Exp Immunol. 2005 May;140(2):249-57. doi: 10.1111/j.1365-2249.2005.02761.x.
Migration of intraepithelial lymphocytes (IELs) into intestinal epithelium is not yet well understood. We established an IEL-cell line from ovalbumin (OVA) 23-3 transgenic (Tg) mice and investigated the effect of antigen stimulation on the dynamic process of IEL migration into small intestinal mucosa. The cell line was a T cell receptor (TCR) alphabeta(+) CD4(+) CD8(-) phenotype, expressing alphaEbeta7 integrin in 90% of cells. Under intravital microscopy, the lined IELs adhered selectively to the microvessels of the intestinal villus tip of the Tg mice. The accumulation of IELs was significantly inhibited by an antibody against beta7-integrin and MAdCAM-1. When IELs were stimulated with OVA, the accumulation was attenuated compared to that of resting cells, with decreased expression of alphaEbeta7 integrin. In Tg mice fed with OVA, the number of IELs which migrated in the villus mucosa was significantly smaller than in the non-fed controls. The preferential migratory capacity of IELs to villus mucosa may be altered by specific antigen stimulations.
上皮内淋巴细胞（IELs）向肠上皮的迁移尚未得到充分了解。我们从卵清蛋白（OVA）23 - 3转基因（Tg）小鼠中建立了IEL细胞系，并研究了抗原刺激对IEL迁移至小肠黏膜动态过程的影响。该细胞系具有T细胞受体（TCR）αβ(+) CD4(+) CD8(-) 表型，90%的细胞表达αEβ7整合素。在活体显微镜下，排列的IELs选择性地黏附于Tg小鼠肠绒毛尖端的微血管。抗β7整合素和黏膜血管地址素细胞黏附分子-1（MAdCAM - 1）的抗体显著抑制了IELs的聚集。当用OVA刺激IELs时，与静息细胞相比，其聚集减弱，αEβ7整合素表达降低。在用OVA喂养的Tg小鼠中，迁移至绒毛黏膜的IELs数量显著少于未喂养的对照组。IELs对绒毛黏膜的优先迁移能力可能会因特定抗原刺激而改变。
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