将新生猪胰岛和支持细胞异种移植到未进行免疫抑制的链脲佐菌素诱导的糖尿病大鼠体内。

Xenotransplantation of neonatal porcine islets and Sertoli cells into nonimmunosuppressed streptozotocin-induced diabetic rats.

作者信息

Wang D Z, Skinner S, Elliot R, Escobar L, Salto-Tellez M, Garkavenko O, Khoo A, Lee K O, Calne R, Isaac J R

机构信息

Department of Surgery, National University of Singapore, Singapore.

出版信息

Transplant Proc. 2005 Jan-Feb;37(1):470-1. doi: 10.1016/j.transproceed.2004.11.057.

DOI:10.1016/j.transproceed.2004.11.057

PMID:15808679

Abstract

The testis has been shown to be a privileged site for transplantation of allogenic islets in rodents, and the testicular cell aggregates are thought to confer this immunologic privilege. Recently, a group in Mexico reported transplantation of cocultured neonatal porcine islets and Sertoli cells resulting in insulin independence in nonimmunosuppressed type 1 diabetes patients. We have transplanted similar islets alone (naked islets) or cocultured islets with Sertoli cells (islet/Sertoli cells) into an omental site and other locations of nonimmunosuppressed, streptozotocin-induced diabetic male Sprague Dawley (SD) rats. Histologic examination showed viable neonatal porcine islets survived in xenografted rodents for at least 2 days, and some glucagon and inhibin stained cells appear to have survived for 4 days posttransplantation. However, histological examination did not demonstrate any difference in xenograft survival in the islets/Sertoli cells mixture compared to naked islets when transplanted into these nonimmunosuppressed diabetic rats.

摘要

睾丸已被证明是啮齿动物同种异体胰岛移植的一个特殊部位，睾丸细胞聚集体被认为赋予了这种免疫特权。最近，墨西哥的一个研究小组报告了共培养的新生猪胰岛和支持细胞的移植，使未接受免疫抑制的1型糖尿病患者实现了胰岛素非依赖。我们已将类似的单独胰岛（裸胰岛）或与支持细胞共培养的胰岛（胰岛/支持细胞）移植到未接受免疫抑制、经链脲佐菌素诱导的糖尿病雄性斯普拉格-道利（SD）大鼠的网膜部位和其他位置。组织学检查显示，移植到异种移植啮齿动物体内的新生猪胰岛至少存活了2天，一些胰高血糖素和抑制素染色的细胞在移植后似乎存活了4天。然而，当移植到这些未接受免疫抑制的糖尿病大鼠体内时，组织学检查并未显示胰岛/支持细胞混合物的异种移植存活率与裸胰岛有任何差异。