Chen B, Liu J, Zhu X, Liu X
Clinical Research Institute, Beijing Friendship Hospital.
Chin Med J (Engl). 1996 Mar;109(3):197-200.
To evaluate the function of alginate poly-L-ysine membrane in protecting porcine to rat islet xenografts.
Newborn porcine islet-like cluster cells (ICC) were obtained by digesting pancreas with type XI collagenase. After 2-3 days' incubation, encapsulated ICC were formed through instrument. In vitro, insulin release and insulin stimulating test was performed. In vivo, encapsulated ICC were transplanted into streptozotocin-induced diabetic rats.
Unlike ICC only (insulin can be tested within 14 days) encapsulated ICC maintained insulin release at certain level for nearly 28 days. High insulin response to 16.7 mM glucose and 10 mM theophylline significantly differed from low insulin response to 5.6 mM or 16.7 mM glucose (P < 0.01). Extended survival of porcine ICC xenografts implanted in diabetic rats had been achieved by encapsulation. After transplantation, rats sustained normal glycemia for average 4 months. The capsules with ICC obtained from experimental group could be stained with neutral red. Insulin release of survival capsules with ICC in response to 16.7 mM glucose and 10 mM theophylline was 3 times more as compared to the insulin response to 5.6 mM glucose. Sections of encapsulated ICC (H&E) showed fairly good cell clusters and the membranes were generally free of fibrosis and host cell adherence.
Artificial membrane can protect discordant islet xenografts from rejection.
评估海藻酸钠聚-L-赖氨酸膜在保护猪胰岛异种移植到大鼠体内时的作用。
用XI型胶原酶消化猪胰腺获取新生猪胰岛样细胞团（ICC）。培养2 - 3天后，通过器械形成包封的ICC。在体外，进行胰岛素释放和胰岛素刺激试验。在体内，将包封的ICC移植到链脲佐菌素诱导的糖尿病大鼠体内。
与仅ICC（14天内可检测到胰岛素）不同，包封的ICC在近28天内将胰岛素释放维持在一定水平。对16.7 mM葡萄糖和10 mM茶碱的高胰岛素反应与对5.6 mM或16.7 mM葡萄糖的低胰岛素反应有显著差异（P < 0.01）。通过包封实现了植入糖尿病大鼠体内的猪ICC异种移植物的长期存活。移植后，大鼠平均维持正常血糖4个月。实验组获得的含ICC的胶囊能用中性红染色。含ICC的存活胶囊对16.7 mM葡萄糖和10 mM茶碱的胰岛素释放量是对5.6 mM葡萄糖胰岛素反应的3倍。包封ICC的切片（苏木精-伊红染色）显示细胞团相当良好，且膜通常无纤维化和宿主细胞黏附。
人工膜可保护异种胰岛移植物免受排斥。
