Sun Ming, Yang Yuru, Li Hong, Su Bin, Lu Yiping, Wei Qiang, Fan Tianyong
Department of Urology of Westchina Hospital of Sichuan University, Chengdu.
Zhong Yao Cai. 2004 Nov;27(11):848-50.
To observe the effect of curcumin on bladder cancer cell line EJ in vitro.
Cell morphology, MTT, flow cytometer, immunocytochemical method for detecting NF-KB, Cyclin D1 were used to observe the effect of 5,10,20 mg/L curcumin on bladder cancer cell line EJ in vitro.
All concentrations curcumin resulted in the growth suppression significantly [Suppression ratio > or = (27.5 + 3.1)%, P < 0.05]. Above 10 mg/L concentrations curcumin induced apoptosis [Apoptosis ratio > or = (14.6 +/- 1.8)%, P < 0.05] and down-regulated of the expression of NF-kappaB [Expression ratio < or = (35.8 +/- 4.2)%, P < 0.05], Cyclin D1 [Expression ratio < or = (29.7 +/- 3.2)%, P < 0.05]. The cell phase arrest induced by curcumin was G1 phase arrest mainly with significant decrease of S phase.
Curcumin can suppress the growth, induce apoptosis of bladder cancer EJ cell in vitro. Its mechanism is related with down-regulations of the expressions of NF-kappaB and Cyclin D1. Curcumin has great potential for the treatment of bladder cancer.
观察姜黄素对膀胱癌细胞系EJ的体外作用。
采用细胞形态学、MTT法、流式细胞仪、免疫细胞化学法检测NF-κB、细胞周期蛋白D1，观察5、10、20mg/L姜黄素对膀胱癌细胞系EJ的体外作用。
各浓度姜黄素均显著抑制细胞生长[抑制率≥(27.5 + 3.1)%，P < 0.05]。10mg/L及以上浓度姜黄素诱导细胞凋亡[凋亡率≥(14.6 ± 1.8)%，P < 0.05]，并下调NF-κB[表达率≤(35.8 ± 4.2)%，P < 0.05]、细胞周期蛋白D1[表达率≤(29.7 ± 3.2)%，P < 0.05]的表达。姜黄素诱导的细胞周期阻滞主要为G1期阻滞，S期显著减少。
姜黄素可体外抑制膀胱癌细胞EJ生长、诱导其凋亡。其机制与下调NF-κB和细胞周期蛋白D1表达有关。姜黄素在膀胱癌治疗方面具有巨大潜力。
