核碱基参与核酶催化作用。
Nucleobase participation in ribozyme catalysis.
作者信息
Zhao Zheng-Yun, McLeod Aileen, Harusawa Shinya, Araki Lisa, Yamaguchi Maho, Kurihara Takushi, Lilley David M J
机构信息
CR-UK Nucleic Acid Structure Research Group, MSI/WTB Complex, University of Dundee, Dundee DD1 5EH, UK.
出版信息
J Am Chem Soc. 2005 Apr 13;127(14):5026-7. doi: 10.1021/ja0502775.
PMID:15810830
Abstract
We constructed a modified form of the VS ribozyme containing an imidazole ring in place of adenine at position 756. The novel ribozyme is active in both cleavage and ligation reactions. The reaction is efficient, although relatively slow. The results are consistent with a role for nucleobase catalysis in the catalytic mechanism of this ribozyme.
摘要
我们构建了一种改良形式的VS核酶，其在756位用咪唑环取代了腺嘌呤。这种新型核酶在切割和连接反应中均具有活性。该反应是有效的，尽管相对较慢。这些结果与核碱基催化在这种核酶催化机制中的作用一致。
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