棉酚对人喉表皮样癌细胞系（HEp-2）细胞毒性作用的体外研究。
An in vitro study of cytotoxic effects of gossypol on human epidermoid larynx carcinoma cell line (HEp-2).
作者信息
Konac Ece, Ekmekci Abdullah, Yurtcu Erkan, Ergun Mehmet Ali
机构信息
Department of Medical Biology and Genetics, Gazi University, Faculty of Medicine, Besevler, Ankara, Turkey.
出版信息
Exp Oncol. 2005 Mar;27(1):81-3.
AIM
The study was aimed on an evaluation of the effect of gossypol at concentration of 25-150 microM on the level of apoptosis in the human epidermoid larynx carcinoma cell line, HEp-2, in vitro.
METHODS
To determine the apoptotic effects of gossypol on growth of human epidermoid larynx carcinoma cell lines (HEp-2 cells), morphological evaluation of the cells and DNA fragmentation analysis were used.
RESULTS
6 h incubation of HEp-2 cells with gossypol at concentrations of 50 microM and 75 microM resulted in the increase of apoptosis rate by 26 and 23%, respectively.
CONCLUSION
This preliminary study may constitute a base for in vivo studies of anticancer properties of gossypol.
目的
本研究旨在评估浓度为25 - 150微摩尔的棉酚对人喉表皮样癌细胞系HEp - 2体外凋亡水平的影响。
方法
为确定棉酚对人喉表皮样癌细胞系（HEp - 2细胞）生长的凋亡作用，采用了细胞形态学评估和DNA片段化分析。
结果
将HEp - 2细胞与浓度为50微摩尔和75微摩尔的棉酚孵育6小时后，凋亡率分别增加了26%和23%。
结论
这项初步研究可能为棉酚抗癌特性的体内研究奠定基础。
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