Keszthelyi S, Acs Z
Department of Botany and Plant Production, Faculty of Animal Science, University of Kaposvár, H-7400 Kaposvár, Hungary.
Acta Biol Hung. 2005;56(1-2):75-81. doi: 10.1556/ABiol.56.2005.1-2.8.
For a molecular genetic study on Hungarian populations of European corn borer L5 stage larvae were collected from 14 places of three different regions of the country (uni- and bivoltine ecotypes). Additionally, the study included larvae from Egypt, too (multivoltine ecotype). Molecular examinations of European corn borer larvae using the study of mitochondrial cytochrome b (cyt b) revealed that by single strand conformation polimorphism (SSCP) the populations found in Hungary represented the same haplotype. Even the Egyptian sample showed no genetic divergence. Some minor deviatons were found in the case of a sample from Székkutas, but that did not prove the genetic divergence of the bivoltine ecotype either, since the other samples of South-East Hungary did not display this kind of genetic variation. On the basis of our investigations it can be said that the univoltine and bivoltine generations, have uniform genetic complements.
为了对匈牙利欧洲玉米螟种群进行分子遗传学研究，从该国三个不同地区的14个地点采集了L5阶段的幼虫（单化性和二化性生态型）。此外，该研究还包括来自埃及的幼虫（多化性生态型）。通过对线粒体细胞色素b（cyt b）的研究对欧洲玉米螟幼虫进行分子检测，结果显示，通过单链构象多态性（SSCP）分析，匈牙利发现的种群代表相同的单倍型。即使是埃及的样本也未显示出遗传差异。在来自塞库塔斯的一个样本中发现了一些微小偏差，但这也没有证明二化性生态型的遗传差异，因为匈牙利东南部的其他样本并未表现出这种遗传变异。根据我们的调查，可以说单化性和二化性世代具有一致的遗传组成。
