Sadasivan E, Cedeno M, Rothenberg S P
Department of Medicine, State University of New York, Health Science Center, Brooklyn.
Biochim Biophys Acta. 1992 May 7;1131(1):91-4. doi: 10.1016/0167-4781(92)90103-7.
An unprocessed pseudogene which is 90% homologous with the cDNA encoding a folate binding protein in KB cells has been cloned from a human genomic library. This pseudogene contains TGA stop codons, base deletions and substitutions and lacks a 5' region. The size of the exons and the intron-exon sites are almost identical to the organization of the gene encoding this protein which has now been characterized from genomic DNA using the polymerase chain reaction with selected primers to the cDNA.
从人类基因组文库中克隆出了一个未加工的假基因，它与KB细胞中编码叶酸结合蛋白的cDNA有90%的同源性。该假基因含有TGA终止密码子、碱基缺失和替换，并且缺少5'区域。外显子的大小和内含子-外显子位点与编码该蛋白的基因结构几乎相同，该基因现已通过使用针对cDNA的选定引物进行聚合酶链反应从基因组DNA中得以鉴定。
