Alexander B, Mathie R T, Ralevic V, Burnstock G
Department of Surgery, Royal Postgraduate Medical School, London, Great Britain.
J Pharmacol Toxicol Methods. 1992 Mar;27(1):17-22. doi: 10.1016/1056-8719(92)90015-s.
An original, isolated dual-perfused rabbit liver preparation was developed for investigations into mechanisms that control the hepatic vascular tone. The hepatic artery (HA) and portal vein (PV) were perfused at constant flows of 0.16 +/- 0.01 and 0.64 +/- 0.05 mL/g/min (n = 5), respectively. Responses of the hepatic arterial and portal venous vascular beds to noradrenaline (NA) were measured as changes in perfusion pressure. Noradrenaline injected directly into the hepatic artery and portal vein produced dose-dependent increases in pressure in the respective vascular beds, the maximum response in the hepatic arterial bed being two to three times greater than that in the portal venous bed. A restricted transmission of vasoconstrictor stimulus between the intrahepatic portal venous and hepatic arterial vasculature was demonstrated. The results demonstrate the suitability of the dual-perfused rabbit liver model for detailed studies of the control of hepatic vascular tone.
为了研究控制肝血管张力的机制，我们开发了一种原始的、孤立的双灌注兔肝制备方法。肝动脉（HA）和门静脉（PV）分别以0.16±0.01和0.64±0.05 mL/g/min的恒定流量灌注（n = 5）。通过测量灌注压力的变化来检测肝动脉和门静脉血管床对去甲肾上腺素（NA）的反应。直接注入肝动脉和门静脉的去甲肾上腺素在各自血管床中产生剂量依赖性的压力升高，肝动脉床的最大反应比门静脉床大两到三倍。结果表明，肝内门静脉和肝动脉血管系统之间的血管收缩刺激传递受限。这些结果证明了双灌注兔肝模型适用于详细研究肝血管张力的控制。
