Browse D J, Benjamin I S, Alexander B
Department of Surgery, King's College School of Medicine and Dentistry, Rayne Institute, London, England, United Kingdom.
J Pharmacol Toxicol Methods. 1994 Oct;32(2):117-22. doi: 10.1016/1056-8719(94)90063-9.
The isolated dual-perfused rabbit liver has been used to characterize hepatic arterial vascular receptors. These hepatic arterial responses are reproducible for 2.5 hr. Further studies in relation to the assessment of portal venous responses using this model require longer periods of perfusion. This study was designed to determine if this model is suitable for the assessment of hepatic arterial and portal venous vascular responses over a 5-hr perfusion period. Hepatic arterial responses were consistent to all agents during 5 hr of perfusion. Portal venous responses to the direct smooth muscle acting agents, sodium nitroprusside and adenosine, were constant, but the vasoconstrictor responses to acetylcholine and adenosine 5'-triphosphate (agents that cause an endothelium-independent vasoconstriction and an endothelium-dependent vasodilatation) were potentiated. Coarse measurements of liver function were also performed and suggested that the liver remained viable for the 5 hr of perfusion. These results suggest a decrease in the ability of the endothelium of the portal venous vasculature to respond to vasoactive substances as duration of perfusion increases. The possible reasons for this are discussed.
分离的双灌注兔肝已被用于表征肝动脉血管受体。这些肝动脉反应在2.5小时内可重复。使用该模型评估门静脉反应的进一步研究需要更长的灌注时间。本研究旨在确定该模型是否适用于评估5小时灌注期内的肝动脉和门静脉血管反应。在5小时的灌注过程中，肝动脉对所有药物的反应是一致的。门静脉对直接作用于平滑肌的药物硝普钠和腺苷的反应是恒定的，但对乙酰胆碱和5'-三磷酸腺苷（引起内皮依赖性血管舒张和内皮依赖性血管收缩的药物）的血管收缩反应增强。还进行了肝功能的粗略测量，结果表明肝脏在5小时的灌注过程中仍保持活力。这些结果表明，随着灌注时间的增加，门静脉血管内皮对血管活性物质的反应能力下降。文中讨论了其可能的原因。
