Cho Sehyung, Yujnovsky Irene, Doi Masao, Sassone-Corsi Paolo
Institut de Génétique et de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, 67404 Illkirch-Strasbourg, France.
Methods Enzymol. 2005;393:469-78. doi: 10.1016/S0076-6879(05)93023-1.
One of the unresolved issues in the field of circadian biology is dissection of the communication pathways between central and peripheral oscillators. We have developed an experimental procedure in which an implant of mouse embryo fibroblasts of a specific genotype can be successfully grafted into a host animal of a different genotype. This methodology provides an excellent tool to study how peripheral clocks are entrained under various physiological settings and the contribution of individual signaling effectors in this process.
昼夜节律生物学领域尚未解决的问题之一是剖析中枢振荡器和外周振荡器之间的通信途径。我们已经开发出一种实验方法，通过该方法可以将特定基因型的小鼠胚胎成纤维细胞植入物成功移植到不同基因型的宿主动物体内。这种方法提供了一个极好的工具，用于研究在各种生理环境下外周生物钟是如何被调节的，以及个体信号效应器在这一过程中的作用。
