Carr M J, McCormack G P, Crowley B
Department of Clinical Microbiology, Sir Patrick Dun Research Laboratory, Trinity College, University of Dublin, Dublin, Ireland.
Clin Microbiol Infect. 2005 May;11(5):366-71. doi: 10.1111/j.1469-0691.2005.01129.x.
Human metapneumovirus (hMPV) is a newly identified paramyxovirus that has been associated with respiratory tract illness in children aged < 5 years, the elderly, and immunocompromised patients. This study determined the frequency of respiratory tract infections (RTIs) associated with hMPV in the Republic of Ireland. Bronchoalveolar lavage (BAL) samples from 168 adult patients and respiratory specimens from 122 children aged < 5 years were collected between September 2003 and May 2004. The virus was detected by reverse-transcription (RT)-PCR using hMPV polymerase (L) and matrix (M)-specific primers in four (2.4%) of 171 BAL specimens obtained from 168 adults. No other respiratory virus was detected in these specimens, and no hMPV RNA was detected in respiratory specimens from children during the same time period. In all four adult cases, two of whom had underlying disease, hMPV was associated with mild, self-limiting upper RTIs. The most common clinical findings included fever (3/4 patients), cough (4/4) and rhinorrhoea (3/4). No patient died as a result of these RTI episodes. Phylogenetic analysis was performed using the amplified regions of the M and fusion (F) genes of hMPV. The Irish isolates belonged to cluster 1B, and did not show a separate Irish sub-lineage.
人偏肺病毒（hMPV）是一种新发现的副粘病毒，与5岁以下儿童、老年人及免疫功能低下患者的呼吸道疾病有关。本研究确定了爱尔兰共和国与hMPV相关的呼吸道感染（RTIs）的发生率。在2003年9月至2004年5月期间，收集了168例成年患者的支气管肺泡灌洗（BAL）样本和122例5岁以下儿童的呼吸道标本。使用hMPV聚合酶（L）和基质（M）特异性引物，通过逆转录（RT）-PCR在从168名成年人获得的171份BAL标本中的4份（2.4%）中检测到该病毒。在这些标本中未检测到其他呼吸道病毒，且在同一时期儿童的呼吸道标本中未检测到hMPV RNA。在所有4例成年病例中，其中2例有基础疾病，hMPV与轻度、自限性上呼吸道感染有关。最常见的临床症状包括发热（3/4患者）、咳嗽（4/4）和流涕（3/4）。这些RTI发作均未导致患者死亡。使用hMPV的M基因和融合（F）基因的扩增区域进行了系统发育分析。爱尔兰分离株属于1B簇，未显示出单独的爱尔兰亚谱系。
