Peng X, Huang J, Ji L
Department of Chemistry, Zhongshan University, 510275 Guangzhou.
Guang Pu Xue Yu Guang Pu Fen Xi. 1999 Dec;19(6):884-5.
The hydrogen bonds between the hydrogen of pyrrols in 5-(2-carboxyphenyl)-10,15,20-triphenylporphyrin and the oxygen of the carboxyl group of amino acid ester were confirmed by UV-Vis spectroscopic titration method and 1H-NMR. When amino acid ester was added to o-TPPCO2H solution in CHCl3, the Soret and Q and red-shifted dramatically and several isobestic points could be observed in UV-Vis spectra. In 1H-NMR, the signal of the hydrogen atoms of pyrrole in o-TPPCO2H (-2.78 ppm) decreased and a new signal in 0.260 ppm emerged, when o-TPPCO2H mixed with L-PheOEt at 1 : 1 (mol/mol).
通过紫外可见光谱滴定法和1H-NMR证实了5-(2-羧基苯基)-10,15,20-三苯基卟啉中吡咯氢与氨基酸酯羧基氧之间的氢键。当将氨基酸酯加入到CHCl3中的邻羧基苯基卟啉（o-TPPCO2H）溶液中时，Soret峰和Q峰显著红移，并且在紫外可见光谱中可观察到几个等吸收点。在1H-NMR中，当o-TPPCO2H与L-苯丙氨酸乙酯以1:1（摩尔/摩尔）混合时，o-TPPCO2H中吡咯氢原子的信号（-2.78 ppm）降低，并且在0.260 ppm处出现一个新信号。
