Kokis V M, Moreira B M, Pellegrino F L P C, Silva M G, Long J B, Bastos C C R, Santos K R N
Department of Medical Microbiology, Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
J Hosp Infect. 2005 May;60(1):19-26. doi: 10.1016/j.jhin.2004.11.019.
A total of 85 Pseudomonas aeruginosa isolates were obtained from October 1999 to April 2000 in a tertiary care hospital in Rio de Janeiro, Brazil. The imipenem susceptibility was evaluated by disk diffusion and agar dilution methods, and the clonal relationship among 67 isolates was examined by macrorestriction profile analysis following pulsed-field gel electrophoresis. Imipenem resistance was observed in 52 (61.2%) isolates. Imipenem-resistant P. aeruginosa isolates were separated into 10 genotypes, 73% of which belonged to genotype A. Identification of a single P. aeruginosa clone with a high rate of imipenem resistance emphasizes the need to control the transmission of this organism among patients.
1999年10月至2000年4月期间，在巴西里约热内卢的一家三级护理医院共获得了85株铜绿假单胞菌分离株。采用纸片扩散法和琼脂稀释法评估亚胺培南敏感性，并通过脉冲场凝胶电泳后的宏观限制性图谱分析检查67株分离株之间的克隆关系。在52株（61.2%）分离株中观察到亚胺培南耐药性。耐亚胺培南铜绿假单胞菌分离株分为10种基因型，其中73%属于基因型A。鉴定出一株亚胺培南耐药率高的铜绿假单胞菌克隆强调了控制该菌在患者之间传播的必要性。
