Shen Yan, Chen Honghui, Li Xianrang, Tang Yi, Zhong Cancan, Liang Peihong, Ren Guomei, Liang Weiguo
Guangzhou Red Cross Hospital of Jinan University Medical College, Guangzhou Institute of Traumatic Surgery, Guangzhou Guangdong 510220, PR China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2005 Mar 15;19(3):229-33.
To investigate the effect of acid, basic fibroblast, growth factor (aFGF, bFGF) and epidermal growth factor (EGF), and their combination on the proliferation of rabbit anterior cruciate ligament (ACL) and medial collateral ligament (MCL) in vitro.
The cells of ACL and MCL were isolated and subcultured from the knee joints of ten-week-old New Zealand white rabbits. The cells were seeded into 96-well corning cluster plates. Three growth factors of different concentration alone or in combination were added into the culture medium respectively, which were 0, 1, 5, 10, 50 and 100 ng/ml for aFGF, bFGF and 0, 1.56, 3.13, 6.25, 12.5, 25 and 50 ng/ml for EGF. The proliferation of the fibroblasts was measured for 48 h with XTT method.
All of the three growth factors alone promoted the cell proliferation of ACL and MCL fibroblasts. The concentration of aFGF had a significant effect on the proliferation of both ACL and MCL fibroblasts. The concentration of 1 ng/ml bFGF and 5 ng/ ml EGF was most effective in promoting the proliferation of ACL, and both bFGF and EGF had a significant effect on MCL. 5 ng/ml aFGF with 50 ng/ml EGF had effect on ACL. 1 ng/ml aFGF with 3.13 ng/ml EGF had effect on MCL.
The three growth factors may promote the cell proliferation of ACL and MCL. These findings suggest that topical application of aFGF, either alone or in combination with EGF may have the potential to promote the proliferation of rabbit ACL and MCL,and aFGF of low concentration in combination with EGF is more effective than single growth factor.
研究酸性成纤维细胞生长因子（aFGF）、碱性成纤维细胞生长因子（bFGF）和表皮生长因子（EGF）及其联合应用对兔前交叉韧带（ACL）和内侧副韧带（MCL）细胞体外增殖的影响。
从10周龄新西兰白兔膝关节分离ACL和MCL细胞并传代培养。将细胞接种于96孔康宁细胞培养板。分别向培养基中添加不同浓度的三种生长因子单独或联合使用，aFGF浓度为0、1、5、10、50和100 ng/ml，bFGF浓度为0、1、5、10、50和100 ng/ml，EGF浓度为0、1.56、3.13、6.25、12.5、25和50 ng/ml。采用XTT法检测成纤维细胞增殖48小时。
三种生长因子单独使用均能促进ACL和MCL成纤维细胞增殖。aFGF浓度对ACL和MCL成纤维细胞增殖均有显著影响。1 ng/ml bFGF和5 ng/ml EGF对促进ACL增殖最有效，bFGF和EGF对MCL均有显著影响。5 ng/ml aFGF与50 ng/ml EGF对ACL有作用。1 ng/ml aFGF与3.13 ng/ml EGF对MCL有作用。
三种生长因子可能促进ACL和MCL细胞增殖。这些发现表明，局部应用aFGF单独或与EGF联合应用可能有促进兔ACL和MCL增殖的潜力，低浓度aFGF与EGF联合应用比单一生长因子更有效。
