Verchot-Lubicz Jeanmarie
Oklahoma State University, Department of Entomology and Plant Pathology, Stillwater, OK 74078, USA.
Mol Plant Microbe Interact. 2005 Apr;18(4):283-90. doi: 10.1094/MPMI-18-0283.
In the last five years, we have gained significant insight into the role of the Potexvirus proteins in virus movement and RNA silencing. Potexviruses require three movement proteins, named triple gene block (TGB)p1, TGBp2, and TGBp3, and the viral coat protein (CP) to facilitate viral cell-to-cell and vascular transport. TGBp1 is a multifunctional protein that has RNA helicase activity, promotes translation of viral RNAs, increases plasmodesmal size exclusion limits, and suppresses RNA silencing. TGBp2 and TGBp3 are membrane-binding proteins. CP is required for genome encapsidation and forms ribonucleoprotein complexes along with TGBp1 and viral RNA. This review considers the functions of the TGB proteins, how they interact with each other and CP, and how silencing suppression might be linked to viral transport. A new model of the mechanism for Potexvirus transport is proposed.
在过去五年中，我们对马铃薯X病毒属蛋白在病毒移动和RNA沉默中的作用有了深入了解。马铃薯X病毒属需要三种移动蛋白，即三基因块（TGB）p1、TGBp2和TGBp3，以及病毒外壳蛋白（CP）来促进病毒的细胞间和维管束运输。TGBp1是一种多功能蛋白，具有RNA解旋酶活性，促进病毒RNA的翻译，增加胞间连丝的大小排阻极限，并抑制RNA沉默。TGBp2和TGBp3是膜结合蛋白。CP是基因组衣壳化所必需的，并与TGBp1和病毒RNA形成核糖核蛋白复合体。本综述探讨了TGB蛋白的功能、它们彼此之间以及与CP的相互作用方式，以及沉默抑制与病毒运输之间可能的联系。并提出了一种马铃薯X病毒属运输机制的新模型。
