斑马鱼中重组信号序列的分析

Analysis of recombination signal sequences in zebrafish.

作者信息

Danilova Nadia

机构信息

Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

出版信息

Mol Immunol. 2005 Jun;42(10):1243-9. doi: 10.1016/j.molimm.2004.11.022. Epub 2005 Jan 5.

DOI:10.1016/j.molimm.2004.11.022

PMID:15829313

Abstract

Recombination signal sequences (RSS) from immunoglobulin and TCRalpha genes of zebrafish were analyzed in comparison with RSS from human and species-specific features were revealed. In contrast to human RSS, in zebrafish RSS from both V(H) and TCRalpha genes the last nonamer position is not conserved. On the contrary, the fourth nonamer position, which is not conserved in human or mouse is conserved in zebrafish. The 12 bp spacers from human and zebrafish RSS contain 9 bp motif resembling nonamer sequence. Spacers in zebrafish 23 bp RSS from both immunoglobulins and TCRalpha contain 7 bp motif also resembling nonamer sequence while corresponding human sequences do not contain analogous motif. RSS are recognized by RAG1 protein, which also has specific features in teleost suggesting co-evolution of RAG1 with corresponding RSS.

摘要

对斑马鱼免疫球蛋白和TCRα基因的重组信号序列（RSS）进行了分析，并与人类的RSS进行比较，揭示了物种特异性特征。与人类RSS不同，斑马鱼V(H)和TCRα基因的RSS中，最后一个九聚体位置不保守。相反，在人类或小鼠中不保守的第四个九聚体位置在斑马鱼中是保守的。人类和斑马鱼RSS的12 bp间隔序列包含一个类似于九聚体序列的9 bp基序。来自免疫球蛋白和TCRα的斑马鱼23 bp RSS中的间隔序列包含一个同样类似于九聚体序列的7 bp基序，而相应的人类序列则不包含类似基序。RSS由RAG1蛋白识别，RAG1在硬骨鱼中也具有特异性特征，这表明RAG1与相应的RSS共同进化。