单核苷酸多态性、谷氨酸和γ-氨基丁酸对大鼠纹状体神经元活动的影响。

Effects of SNP, GLU and GABA on the neuronal activity of striatum nucleus in rats.

作者信息

Liu Chun-Na, Liu Xinyu, Gao Dongming, Li Shengnan

机构信息

The Laboratory of Reproductive Medicine, Department of Pharmacology, Nanjing Medical University, Nanjing 210029, China.

出版信息

Pharmacol Res. 2005 Jun;51(6):547-51. doi: 10.1016/j.phrs.2005.02.003.

DOI:10.1016/j.phrs.2005.02.003

PMID:15829435

Abstract

This study investigated the activity of nitric oxide (NO) in the striatum (STR) for a further comprehension of the pathogenesis of Parkinson's disease (PD). Microiontophoresis was used to observe the effects of sodium nitroprusside (SNP), L-glutamic acid (GLU) and gamma-aminobutyric acid (GABA) on STR neurons' firing rates. It was observed that 77.27% (51/66) of the tested STR neurons were excited by SNP. This excitatory effect could be antagonized by the NO synthase (NOS) inhibitor, N(G)-nitro-L-arginine methyl ester (L-NAME). During the microiontophoresis of GLU, the excitatory firing of STR neurons was also attenuated by addition of L-NAME while SNP application could enhance the excitation of the neurons. On the other hand, in the presence of GABA, SNP still excited the tested STR neurons. These results demonstrated that NOergic, GLUergic and GABAergic co-existed in the same STR neurons. NOergic and GLUergic were excitatory whereas GABAergic was inhibitory on the firing activity in STR neurons.

摘要

本研究调查了纹状体（STR）中一氧化氮（NO）的活性，以进一步了解帕金森病（PD）的发病机制。采用微离子电泳法观察硝普钠（SNP）、L-谷氨酸（GLU）和γ-氨基丁酸（GABA）对STR神经元放电频率的影响。结果发现，77.27%（51/66）的受试STR神经元被SNP兴奋。这种兴奋作用可被一氧化氮合酶（NOS）抑制剂N（G）-硝基-L-精氨酸甲酯（L-NAME）拮抗。在GLU微离子电泳过程中，加入L-NAME也可减弱STR神经元的兴奋性放电，而应用SNP可增强神经元的兴奋。另一方面，在GABA存在的情况下，SNP仍能兴奋受试的STR神经元。这些结果表明，NO能、GLU能和GABA能在同一STR神经元中共存。NO能和GLU能具有兴奋性，而GABA能对STR神经元的放电活动具有抑制性。