Friberg J A, Weathers P J, Gibson D G
Department of Biology and Biotechnology, Worcester Polytechnic Institute, Massachusetts 01609.
In Vitro Cell Dev Biol. 1992 Mar;28A(3 Pt 1):215-7. doi: 10.1007/BF02631095.
We report the first use of nutrient mist bioreactor (NMB) technology to culture animal cells. The nutrient mist approximated the amebocyte stem tissue's natural environment, which is a thin layer of fluid in the gill leaflets of the horseshoe crab Limulus polyphemus. NMB culture was tried in an attempt to increase production of amebocytes, which are the source of the Limulus Amebocyte Lysate (LAL), the basis for a sensitive and commercially valuable endotoxin assay. Amebocyte growth in the nutrient mist bioreactor is comparable to growth in liquid medium. However, the current design of the bioreactor presents problems for primary cultures such as ours where a pyrogen-free environment is necessary and fungal decontamination is difficult.
我们报告了首次使用营养雾生物反应器（NMB）技术培养动物细胞的情况。营养雾模拟了变形细胞干细胞组织的自然环境，即马蹄蟹美洲鲎鳃小叶中的一层薄流体。尝试进行NMB培养是为了增加变形细胞的产量，变形细胞是鲎试剂（LAL）的来源，而鲎试剂是一种灵敏且具有商业价值的内毒素检测方法的基础。营养雾生物反应器中变形细胞的生长与在液体培养基中的生长相当。然而，生物反应器的当前设计给像我们这样的原代培养带来了问题，在原代培养中需要无热原环境且真菌去污困难。
