Minezaki K K, Nakazawa H, Shinozaki Y, Ichimori K, Okino H
Department of Physiology 2, School of Medicine, Tokai University, Kanagawa, Japan.
Heart Vessels. 1992;7(1):31-6. doi: 10.1007/BF01745865.
We studied the concomitant effects of scavengers of reactive oxygen species (ROS) on both cardiac function and the incidence of arrhythmias. Isolated rat heart was perfused with a working mode paced at 300 beats/min. The left coronary artery was occluded for 5, 7, 15, or 60 min and reperfused thereafter for 30 min. Superoxide dismutase and catalase were infused from 5 min prior to reperfusion to the end of reperfusion in the scavenger treatment group. In the 60-min ischemia group with scavenger treatment, the cardiac output was significantly higher than that in the untreated group at both 10 and 30 min of reperfusion (P less than 0.01). In the 15-min ischemia group with scavenger treatment, the cardiac output showed a tendency toward a higher value than that in the untreated group. The incidence of reperfusion arrhythmias occurring after a short ischemic time (5, 7, or 15 min) were similar in the scavenger treated and untreated groups; but, with a preceding ischemia of 60 min, the incidence of ventricular tachycardia was higher in the scavenger treated group than in the untreated group (P less than 0.02). In conclusion, scavengers improved contractile dysfunction but did not attenuate the incidence of arrhythmias.
我们研究了活性氧（ROS）清除剂对心脏功能和心律失常发生率的协同作用。将离体大鼠心脏以每分钟300次的起搏频率进行工作模式灌注。左冠状动脉闭塞5、7、15或60分钟，然后再灌注30分钟。在清除剂治疗组中，从再灌注前5分钟至再灌注结束时输注超氧化物歧化酶和过氧化氢酶。在清除剂治疗的60分钟缺血组中，再灌注10分钟和30分钟时的心输出量均显著高于未治疗组（P<0.01）。在清除剂治疗的15分钟缺血组中，心输出量有高于未治疗组的趋势。在短时间缺血（5、7或15分钟）后发生的再灌注心律失常的发生率在清除剂治疗组和未治疗组中相似；但是，在先前缺血60分钟的情况下，清除剂治疗组的室性心动过速发生率高于未治疗组（P<0.02）。总之，清除剂改善了收缩功能障碍，但并未降低心律失常的发生率。
