Lewis J A, Dolan J W, Snyder L R, Molnar I
LC Resources Inc., Walnut Creek, CA 94596.
J Chromatogr. 1992 Feb 21;592(1-2):197-208. doi: 10.1016/0021-9673(92)85086-9.
The optimization of reversed-phase high-performance liquid chromatographic separation by the simultaneous variation of pH and solvent strength (%B) was studied for acidic (substituted benzoic acids) and basic samples (substituted anilines). The combination of these two variables was expected to be more useful than either variable alone. This proved to be the case for the benzoic acid sample, but not for the aniline sample. Column plate numbers were also studied for each sample and as a function of pH. With the exception of one compound (3,5-dimethylaniline) in one particular pH range (3.0-4.5), plate numbers of 12,000-20,000 were observed for each sample.
通过同时改变pH值和溶剂强度（%B）来优化反相高效液相色谱分离，对酸性样品（取代苯甲酸）和碱性样品（取代苯胺）进行了研究。预计这两个变量的组合比单独使用任何一个变量都更有用。对于苯甲酸样品确实如此，但对于苯胺样品并非如此。还研究了每个样品的柱板数以及作为pH函数的柱板数。除了在一个特定pH范围（3.0 - 4.5）内的一种化合物（3,5 - 二甲基苯胺）外，每个样品的柱板数在12,000 - 20,000之间。
