Wistuba Dorothee, Banspach Leena, Schurig Volker
Institute of Organic Chemistry, University of Tübingen, D-72076 Tübingen, Germany.
Electrophoresis. 2005 May;26(10):2019-26. doi: 10.1002/elps.200410251.
By an on-column sol-gel process, a chiral monolithic stationary phase was prepared by the fusion of permethyl-beta-cyclodextrin-silica (Chira-Dex-silica) particles and by linking them to the internal capillary wall. The resulting monolith is stable toward voltage (30 kV) and pressure (300 bar) and possesses a high efficiency (up to 100,000 theoretical plates per meter). Efficient enantiomeric separation of various chiral compounds by pressure-supported capillary electrochromatography (CEC) was achieved. When comparing this method to capillary liquid chromatography (LC) employing the same column in an unified equipment, CEC shows a twofold higher column efficiency at comparable elution times and hence better resolution factors.
通过柱上溶胶-凝胶法，通过全甲基化β-环糊精-二氧化硅（Chira-Dex-二氧化硅）颗粒的融合并将它们连接到内部毛细管管壁上，制备了一种手性整体固定相。所得整体对电压（30 kV）和压力（300 bar）稳定，并具有高效率（每米高达100,000理论塔板数）。通过压力支持的毛细管电色谱（CEC）实现了各种手性化合物的高效对映体分离。当在统一设备中使用同一根柱子将该方法与毛细管液相色谱（LC）进行比较时，CEC在相当的洗脱时间下显示出两倍高的柱效率，因此具有更好的分离因子。
