抑制性基序杀伤细胞免疫球蛋白样受体KIR2DL1在盆腔子宫内膜异位症女性自然杀伤细胞中的表达增加。
Expression of inhibitory-motif killer immunoglobulin-like receptor, KIR2DL1, is increased in natural killer cells from women with pelvic endometriosis.
作者信息
Matsuoka Satomi, Maeda Nagamasa, Izumiya Chiaki, Yamashita Chika, Nishimori Yoshiko, Fukaya Takao
机构信息
Department of Obstetrics and Gynecology, Kochi Medical School, Kochi, 783-8505, Japan.
出版信息
Am J Reprod Immunol. 2005 May;53(5):249-54. doi: 10.1111/j.1600-0897.2005.00271.x.
PROBLEM
We investigated inhibitory and activation motif expression of killer immunoglobulin-like receptor (KIR) by natural killer (NK) cells, which may be pathogenetically involved in endometriosis.
METHOD OF STUDY
We compared cells from 24 Japanese women laparoscopically diagnosed with endometriosis, to cells from 25 women with other laparoscopic diagnoses. KIR expression by NK cells was assessed in peripheral blood (PB) and peritoneal fluid (PF) by flow cytometry. Intracellular immunoreceptor tyrosine-based (IT) inhibitory and activation motifs (ITIM and ITAM) of KIR in PB was assessed by Western blotting.
RESULTS
ITIM-KIR expression by PB NK cells was significantly and similarly greater than ITAM-KIR expression in women with and without endometriosis. Percentages of CD56(+) NK cells in PB and PF did not differ significantly between women with and without endometriosis; however, the percentage of CD158a(+) cells among CD56(+) NK cells in PB and PF was significantly higher in women with than without endometriosis.
CONCLUSIONS
ITIM-KIR expressing NK cells might confer tolerance to peritoneal endometriotic implants.
问题
我们研究了自然杀伤（NK）细胞杀伤性免疫球蛋白样受体（KIR）的抑制性和激活性基序表达，其可能在发病机制上与子宫内膜异位症有关。
研究方法
我们将24例经腹腔镜诊断为子宫内膜异位症的日本女性的细胞与25例经腹腔镜诊断为其他疾病的女性的细胞进行比较。通过流式细胞术评估外周血（PB）和腹腔液（PF）中NK细胞的KIR表达。通过蛋白质印迹法评估PB中KIR的细胞内基于免疫受体酪氨酸的（IT）抑制性和激活性基序（ITIM和ITAM）。
结果
无论有无子宫内膜异位症，PB NK细胞的ITIM-KIR表达均显著且相似地高于ITAM-KIR表达。有无子宫内膜异位症的女性PB和PF中CD56(+) NK细胞的百分比无显著差异；然而，有子宫内膜异位症的女性PB和PF中CD56(+) NK细胞中CD158a(+)细胞的百分比显著高于无子宫内膜异位症的女性。
结论
表达ITIM-KIR的NK细胞可能赋予对腹膜子宫内膜异位种植体的耐受性。
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