Aydin Selcen, Yargicoglu Piraye, Derin Narin, Aliciguzel Yakup, Abidin Ismail, Agar Aysel
Akdeniz University, Faculty of Medicine, Department of Biophysics, Arapsuyu, 07070 Antalya, Turkey.
Food Chem Toxicol. 2005 Jul;43(7):1093-101. doi: 10.1016/j.fct.2005.02.014.
Stress and sulfite can stimulate numerous pathways leading to an increased production of free radicals which generate a peroxidation cascade producing lipid peroxidation, protein oxidation, DNA damage and cell death, and contribute to the occurrence of pathologic conditions. The purpose of our study was to investigate the effects of stress and sulfite on visual evoked potentials (VEPs) and to examine the relationship between lipid peroxidation and VEP changes. Forty male wistar rats, aged three months were used. They were equally divided into four groups: control (C), the group exposed to restraint stress (R), the group treated with sulfite (S) and the group exposed to stress and treated with sulfite (RS). Chronic restraint stress was applied for 21 days (1h/day) and sodium metabisulfite (520 mg/kg/day) was given by gavage for the same period. All latencies of VEP components were prolonged in the R, S and RS groups with respect to the C group. Brain and retina TBARS levels were found to be increased in those groups compared to the C group. Our results strongly suggest that the latency prolongations of all VEP components may have resulted from the enhancement of lipid peroxidation. Correlation analysis between brain and retina TBARS levels and VEP latencies also support this conclusion. Our data in regard to latency prolongations of all VEP components in the stress group exposed to sulfite, reflected an additive effect of sulfite toxicity on VEP components. Therefore it could be suggested that chronic exposure to stress and sulfite together is associated with a considerable health risk.
应激和亚硫酸盐可刺激多种途径，导致自由基生成增加，进而引发过氧化级联反应，产生脂质过氧化、蛋白质氧化、DNA损伤和细胞死亡，并促使病理状况的发生。我们研究的目的是探讨应激和亚硫酸盐对视诱发电位（VEP）的影响，并研究脂质过氧化与VEP变化之间的关系。选用40只3月龄雄性Wistar大鼠，将它们平均分为四组：对照组（C）、暴露于束缚应激的组（R）、用亚硫酸盐处理的组（S）以及暴露于应激并用亚硫酸盐处理的组（RS）。慢性束缚应激持续21天（每天1小时），同期通过灌胃给予焦亚硫酸钠（520毫克/千克/天）。与C组相比，R组、S组和RS组的所有VEP成分潜伏期均延长。与C组相比，这些组的脑和视网膜丙二醛水平升高。我们的结果强烈表明，所有VEP成分潜伏期的延长可能是脂质过氧化增强所致。脑和视网膜丙二醛水平与VEP潜伏期之间的相关性分析也支持这一结论。我们关于暴露于亚硫酸盐的应激组中所有VEP成分潜伏期延长的数据，反映了亚硫酸盐毒性对VEP成分的叠加效应。因此，可以认为长期同时暴露于应激和亚硫酸盐会带来相当大的健康风险。
