50赫兹磁场（0.1毫特斯拉）会改变植入后早期小鼠胚胎的c-fos信使核糖核酸表达以及妊娠小鼠的血清雌二醇水平。
50-Hz magnetic field (0.1-mT) alters c-fos mRNA expression of early post implantation mouse embryos and serum estradiol levels of gravid mice.
作者信息
Shi Yijun, Bao Xiuqi, Huo Xiaolin, Shen Zheng, Song Tao
机构信息
Bioelectromagnetic Lab, Institute of Electrical Engineering, Chinese Academy of Sciences, Beijing, China.
出版信息
Birth Defects Res B Dev Reprod Toxicol. 2005 Apr;74(2):196-200. doi: 10.1002/bdrb.20036.
BACKGROUND
We exposed pregnant mice to magnetic or sham fields for 24 hr/day during Gestation Day (GD) 5.5-8.5 to study the effects of 50-Hz 0.1-mT sinusoidal magnetic fields on early pregnancy in mouse embryos and mice.
METHODS
Mice were sacrificed on GD 8.5. Embryos and blood samples were collected. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of embryo c-fos mRNA. The blood samples were tested through radioimmunoassay for serum estradiol levels and general examinations.
RESULTS
We found that the expression of c-fos mRNA of embryos under exposure was enhanced. We reported that 50-Hz 0.1-mT magnetic exposure induced the decline of serum estradiol levels of pregnancy mice on GD 8.5. General examinations of blood including white blood cell (WBC) count, red blood cell (RBC) count, and hemoglobin (HGB) did not show significant differences between the exposure group and the control group (sham exposure).
CONCLUSIONS
Under the experimental conditions, 50-Hz 0.1-mT sinusoidal magnetic fields affected the development of early pregnancy mouse embryos and the dams to some extent.
背景
我们在妊娠第5.5 - 8.5天期间，将怀孕小鼠每天暴露于磁场或假磁场中24小时，以研究50赫兹0.1毫特斯拉正弦磁场对小鼠胚胎和小鼠早期妊娠的影响。
方法
在妊娠第8.5天处死小鼠。收集胚胎和血液样本。采用逆转录聚合酶链反应（RT-PCR）检测胚胎c-fos mRNA的表达。通过放射免疫分析法检测血液样本中的血清雌二醇水平并进行常规检查。
结果
我们发现暴露组胚胎的c-fos mRNA表达增强。我们报告称，50赫兹0.1毫特斯拉的磁场暴露导致妊娠第8.5天的怀孕小鼠血清雌二醇水平下降。包括白细胞（WBC）计数、红细胞（RBC）计数和血红蛋白（HGB）在内的血液常规检查在暴露组和对照组（假暴露）之间未显示出显著差异。
结论
在实验条件下，50赫兹0.1毫特斯拉正弦磁场在一定程度上影响了早期妊娠小鼠胚胎和母鼠的发育。
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