Asimeng E J, Mutinga M J
Medical Vectors Research Programme, International Centre of Insect Physiology and Ecology, Nairobi, Kenya.
J Am Mosq Control Assoc. 1992 Mar;8(1):86-8.
A large number of source materials were collected for isolating entomopathogenic bacteria from larval mosquito habitats in Kirinyaga District, Kenya. Mosquito-toxic bacteria were included among the numerous types of microorganisms isolated from the habitats. The pathogenic isolates shared common structural characteristics; they were gram-positive, spore-forming bacilli that produced parasporal inclusions conferring broad-spectrum larvicidal activity against Anopheles, Culex and Aedes mosquitoes. Based on structural and growth characteristics, coupled with larvicidal activity, the pathogenic isolates were tentatively identified as variants of Bacillus thuringiensis. Although the collection consisted of a variety of items including soil, silt and mud, the most productive materials were larval bodies. Using healthy mosquito larvae held in a fully permeable plastic bottle, a baiting technique was developed as a means of recovering bacteria from the environment.
为了从肯尼亚基里尼亚加区的幼虫蚊虫栖息地分离昆虫病原细菌，收集了大量的源材料。从这些栖息地分离出的众多微生物类型中包括对蚊子有毒的细菌。致病分离株具有共同的结构特征；它们是革兰氏阳性、形成芽孢的杆菌，产生伴孢晶体，对按蚊、库蚊和伊蚊具有广谱杀幼虫活性。根据结构和生长特征，结合杀幼虫活性，这些致病分离株被初步鉴定为苏云金芽孢杆菌的变种。虽然收集的材料包括土壤、淤泥和泥浆等各种物品，但最有成效的材料是幼虫尸体。利用放在完全可渗透塑料瓶中的健康蚊虫幼虫，开发了一种诱饵技术，作为从环境中回收细菌的一种方法。
