电离辐射对大鼠腮腺交感神经功能的影响。

Effect of ionizing radiation on sympathetic nerve function in rat parotid glands.

作者信息

Kohn W G, Grossman E, Fox P C, Armando I, Goldstein D S, Baum B J

机构信息

Clinical Investigations and Patient Care Branch, National Institute of Dental Research, Bethesda, Maryland 20892.

出版信息

J Oral Pathol Med. 1992 Mar;21(3):134-7. doi: 10.1111/j.1600-0714.1992.tb00997.x.

DOI:10.1111/j.1600-0714.1992.tb00997.x

PMID:1583597

Abstract

Ionizing radiation (IR) irreversibly damages salivary glands. The pathologic mechanism is unknown. Previously we reported that parotid serous acinar cells may not be the primary site of damage by IR. The purpose of this study was to determine if IR alters sympathetic nerve function in rat parotid glands. Male adult rats received a single dose of radiation (20 Gy) to the head and neck. Three days after IR, parotid saliva secretion induced by norepinephrine (NE) was completely blocked. Catecholamine uptake and metabolism were studied by injecting [3H] dopamine ([3H]DA) into irradiated rats, as a bolus. After 60 min, animals were sacrificed and the parotid gland, submandibular gland, and left ventricle removed. Tissue contents of [3H]DA and [3H]NE, identified by HPLC, were unaffected by IR. The results indicate that IR abolishes acinar responsiveness to NE without affecting parotid sympathetic nerve function.

摘要

电离辐射（IR）会对唾液腺造成不可逆的损伤。其病理机制尚不清楚。此前我们报道过，腮腺浆液性腺泡细胞可能不是IR损伤的主要部位。本研究的目的是确定IR是否会改变大鼠腮腺中的交感神经功能。成年雄性大鼠接受单次头部和颈部辐射（20 Gy）。IR后三天，去甲肾上腺素（NE）诱导的腮腺唾液分泌被完全阻断。通过向受辐照大鼠一次性注射[3H]多巴胺（[3H]DA）来研究儿茶酚胺的摄取和代谢。60分钟后，处死动物并取出腮腺、颌下腺和左心室。通过高效液相色谱法鉴定的[3H]DA和[3H]NE的组织含量不受IR影响。结果表明，IR消除了腺泡对NE的反应性，而不影响腮腺交感神经功能。