Kang J H, Lee M S
Department of Microbiology, Pukyong National University, Busan, South Korea.
J Appl Microbiol. 2005;98(5):1169-76. doi: 10.1111/j.1365-2672.2005.02556.x.
To partially characterize the bacteriocin produced by the GM-1 strain of Enterococcus faecium, isolated from the faeces of a newborn human infant.
The bacteriocin produced by E. faecium GM-1 showed a broad spectrum of activity against indicator strains of Escherichia coli, Staphylococcus aureus, Vibrio spp., Salmonella typhimurium, Listeria monocytogenes, Lactobacillus acidophilus, and Streptococcus thermophilus. Treatment of the GM-1 bacteriocin with proteolytic enzymes reduced its inhibitory activities. The bacteriocin was stable at 100 degrees C for 20 min and displayed inhibitory activity at neutral pH. The optimal production of bacteriocin from E. faecium GM-1 was obtained when the culture conditions were pH 6.0-6.5 and 35-40 degrees C. The inhibitory activity of the bacteriocin was not substantially changed by the use of different carbon sources in the media, except when galactose was substituted for glucose. The use of a sole nitrogen source caused a decrease in inhibitory activity. A bacteriocin gene similar to enterocin P was identified from the total DNA of E. faecium GM-1 by PCR and direct sequencing methods.
E. faecium GM-1, which was isolated from the faeces of a newborn baby, produces an enterocin P-like bacteriocin with inhibitory activity against Gram-positive and Gram-negative bacteria, including food-borne pathogens.
E. faecium GM-1, isolated from infant faeces, produces a new bacteriocin that is similar to enterocin P. This bacteriocin is heat stable and has a broad antibacterial spectrum that includes both Gram-positive and Gram-negative bacteria.
对从一名新生儿粪便中分离出的屎肠球菌GM - 1菌株产生的细菌素进行部分特性鉴定。
屎肠球菌GM - 1产生的细菌素对大肠杆菌、金黄色葡萄球菌、弧菌属、鼠伤寒沙门氏菌、单核细胞增生李斯特菌、嗜酸乳杆菌和嗜热链球菌等指示菌株表现出广泛的活性。用蛋白水解酶处理GM - 1细菌素会降低其抑制活性。该细菌素在100℃下20分钟稳定,在中性pH下具有抑制活性。当培养条件为pH 6.0 - 6.5和35 - 40℃时,屎肠球菌GM - 1产生细菌素的产量最佳。除了用半乳糖替代葡萄糖外,培养基中使用不同的碳源对细菌素的抑制活性没有实质性影响。使用单一氮源会导致抑制活性下降。通过PCR和直接测序方法从屎肠球菌GM - 1的总DNA中鉴定出一个与肠球菌素P相似的细菌素基因。
从新生儿粪便中分离出的屎肠球菌GM - 1产生一种类似肠球菌素P的细菌素,对革兰氏阳性和革兰氏阴性细菌包括食源性病原体具有抑制活性。
从婴儿粪便中分离出的屎肠球菌GM - 1产生一种与肠球菌素P相似的新型细菌素。这种细菌素热稳定,具有包括革兰氏阳性和革兰氏阴性细菌在内的广泛抗菌谱。