围产期铜缺乏后，大鼠脑铁浓度较低。
Rat brain iron concentration is lower following perinatal copper deficiency.
作者信息
Prohaska Joseph R, Gybina Anna A
机构信息
Department of Biochemistry and Molecular Biology, University of Minnesota, Duluth, Minnesota 55812, USA.
出版信息
J Neurochem. 2005 May;93(3):698-705. doi: 10.1111/j.1471-4159.2005.03091.x.
Experiments performed with Holtzman rats demonstrated that brain iron (Fe) was lower by postnatal day 13 (P13) in pups born and nursed by dams that began copper-deficient (-Cu) treatment at embryonic day 7. Transcardial perfusion of P24-P26 males and females to remove blood Fe contamination revealed that brain Fe was still 20% lower in -Cu than +Cu rats. Estimated blood content of brain for -Cu rats was greater than for +Cu rats; for all groups, values ranged between 0.43 and 1.03%. Using group-specific data and regression analyses, r = 0.99, relating blood Fe to hemoglobin, brain Fe in non-perfused rats in a replicate study was lower by 33% at P13 and 39% at P24 in -Cu rats. Brain extracts from these rats and from P50 rats from a post-weaning model were compared by immunoblotting for transferrin receptor (TfR1). P24 brain -Cu/+Cu TfR1 was 3.08, suggesting that brains of -Cu rats were indeed Fe deficient. This ratio in P13 rats was 1.44, p < 0.05. No change in P50 -Cu rat brain TfR1 or Fe content was detected despite a 50% reduction in plasma Fe. The results suggest that brain Fe accumulation depends on adequate Cu nutriture during perinatal development.
对霍尔茨曼大鼠进行的实验表明，在胚胎第7天开始接受缺铜（-Cu）处理的母鼠所生并哺育的幼崽中，到出生后第13天（P13）时脑铁（Fe）含量较低。对P24 - P26的雄性和雌性大鼠进行经心灌注以去除血液中铁的污染，结果显示，-Cu组大鼠的脑铁含量仍比+Cu组大鼠低20%。-Cu组大鼠脑的估计血液含量高于+Cu组大鼠；所有组的值在0.43%至1.03%之间。在一项重复研究中，使用特定组的数据和回归分析（r = 0.99，将血液铁与血红蛋白相关联），-Cu组大鼠在P13时未灌注大鼠的脑铁含量低33%，在P24时低39%。通过对转铁蛋白受体（TfR1）进行免疫印迹，比较了这些大鼠以及断奶后模型中P50大鼠的脑提取物。P24时脑的-Cu / +Cu TfR1为3.08，表明-Cu组大鼠的脑确实缺铁。P13大鼠的这一比值为1.44，p < 0.05。尽管血浆铁减少了50%，但未检测到P50 -Cu组大鼠脑TfR1或铁含量的变化。结果表明，脑铁的积累取决于围产期发育期间充足的铜营养。
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