N-DEVD-N'-吗啉羰基罗丹明110：用于基于细胞的凋亡检测的新型半胱天冬酶-3荧光底物。
N-DEVD-N'-morpholinecarbonyl-rhodamine 110: novel caspase-3 fluorogenic substrates for cell-based apoptosis assay.
作者信息
Wang Zhi-Qiang, Liao Jinfang, Diwu Zhenjun
机构信息
Department of Reagent and Assay Development, Molecular Devices Corporation, 1311 Orleans Drive, Sunnyvale, CA 94089, USA.
出版信息
Bioorg Med Chem Lett. 2005 May 2;15(9):2335-8. doi: 10.1016/j.bmcl.2005.02.081.
PMID:15837320
Abstract
A novel caspase-3 substrate N-Ac-DEVD-N'-MC-R110, which is a fluorogenic substrate cleavable in a single step, has been prepared. It has a significantly higher enzyme turnover rate and sensitivity for detecting caspase-3 activity both in solution and living cells than existing fluorogenic substrates.
摘要
一种新型的半胱天冬酶-3底物N-乙酰-天冬氨酸-谷氨酸-缬氨酸-天冬氨酸-N'-甲基香豆素-罗丹明110已被制备出来，它是一种可一步切割的荧光底物。与现有的荧光底物相比，它在检测溶液和活细胞中的半胱天冬酶-3活性时具有显著更高的酶转换率和灵敏度。
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