T11TS/S-LFA3诱导脑肿瘤细胞凋亡：一种通过任意引物PCR表征凋亡相关基因变化的新方法。

T11TS/S-LFA3 induces apoptosis of the brain tumor cells: a new approach to characterise the apoptosis associated genetic changes by arbitrarily primed-PCR.

作者信息

Mukherjee Joydeep, Ghosh Anirban, Sarkar Susobhan, Mazumdar Malabika, Sarkar Pallab, Duttagupta Ashish Kumar, Chaudhuri Swapna

机构信息

Cellular and Molecular Immunology Lab, Department of Physiology, Dr B. C. Roy Post Graduate Institute of Basic Medical Sciences, Institute of Post Graduate Medical Education and Research, Kolkata, West Bengal, India.

出版信息

Cancer Lett. 2005 May 10;222(1):23-38. doi: 10.1016/j.canlet.2004.09.014.

DOI:10.1016/j.canlet.2004.09.014

PMID:15837538

Abstract

Genetic alterations in ethyl nitrosourea (ENU) induced brain tumor model were analysed by simple PCR based technique with arbitrary primers. T11TS/SLFA3 was established previously as a potent immune stimulator with antineoplastic property in experimental glioma model. The goal of this study was to reveal whether T11TS induces apoptosis of the neural neoplastic cell and to decipher the DNA polymorphism level of the cells undergoing apoptosis. The results clearly establish the apoptogenic role of T11TS/S-LFA3 and along with the detection of cancer associated genomic instability, AP-PCR analysis is useful for the detection of DNA level fragmentation, a unique feature of apoptosis.

摘要

通过基于简单PCR的任意引物技术分析了乙基亚硝基脲（ENU）诱导的脑肿瘤模型中的基因改变。T11TS/SLFA3先前被确立为在实验性胶质瘤模型中具有抗肿瘤特性的强效免疫刺激剂。本研究的目的是揭示T11TS是否诱导神经肿瘤细胞凋亡，并解读正在经历凋亡的细胞的DNA多态性水平。结果清楚地证实了T11TS/S-LFA3的凋亡诱导作用，并且连同癌症相关基因组不稳定性的检测，AP-PCR分析对于检测DNA水平片段化（凋亡的一个独特特征）是有用的。