Kawazoe Yumi, Shiba Toshikazu, Yamamoto Masahito, Ohuchi Azuma
Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
Proc IEEE Comput Soc Bioinform Conf. 2002;1:314-20.
The personal information encoded in genomic DNA should not be made available to the public. With the increasing discoveries of new genes, it has become necessary to establish a security system for personal genome information. Although many security systems that are applied for electrical information in computers have been developed and established, there is no security system for information at DNA level. In this paper, we describe a new security system for information encoded within DNA. The original genomic DNA was mixed with many kinds of dummy DNAs (mixtures of natural and/or artificial DNAs) resulting in the masking of the original information. Using these dummy molecules, we succeeded to completely 'lock'the original genome information. If this information must be 'unlocked', it can be extracted and analyzed by a removal of dummy DNAs using molecular tagging techniques or by selective amplification using key primers.
基因组DNA中编码的个人信息不应向公众公开。随着新基因的发现日益增多，建立个人基因组信息安全系统变得十分必要。尽管已开发并建立了许多应用于计算机电子信息的安全系统，但在DNA层面尚无信息安全系统。在本文中，我们描述了一种用于DNA编码信息的新安全系统。原始基因组DNA与多种虚拟DNA（天然和/或人工DNA的混合物）混合，从而掩盖了原始信息。利用这些虚拟分子，我们成功地完全“锁定”了原始基因组信息。如果必须“解锁”此信息，可以通过使用分子标记技术去除虚拟DNA或使用关键引物进行选择性扩增来提取和分析。
